This is a chart provided by IDEXX which we go by for breeding. Many breedings are done far too early and result in misses. We would like to minimize misses based on timing. Please follow this chart and give to your veterinarian when planning for a breeding. Please invest in progesterone numbers so you have the best chance to get a litter!
You can usually wait to do the first progesterone number on the 8th day. Do not stop testing until you've reached a number over 5.
Below 1.0 ng/ml - no ovarian activity - ACTION: Test every 3 or 4 days
1.0 to 2.0 ng/ml - rise indicates active ovary - ACTION: test in 2 to 3 days
2.0 to 4.9 ng/ml - active ovary - ACTION - Test every 24 hours until you determine 5.0
5.0 ng/ml OVULATION
5 to 6 ng/ml - ACTION - Breed in 2 to 3 days
6 to 11 ng/ml - ACTION - BREED in 1 to 2 days
11 to 19 ng/ml - ACTION - BREED TODAY
OVER 19 ng/ml - ACTION - BREED IMMEDIATELY if vaginal smear is OK and bitch is still receptive. This is considered LATE if you still have to ship semen. Hope for the best.
Not much can be determined by the numbers after 5, but you should not determine a breeding date until you get a number 5 or over.